Effect of total saponins of Panax notoginseng on neuroregeneration in subventricle zone of rats with global cerebral ischemia / 中草药

ABSTRACT

Objective: To explore the effect of total saponins of Panax notoginseng (TSPN) on the neuroregeneration in subventricle zone (SVZ) in rats with global cerebral ischemia. Methods: Using four-vessel occlusion method to build the global cerebral ischemia model. Rats were divided into Sham group, vehicle group, and TSPN group. The rats in TSPN group were ip administered with TSPN 30 min post-brain ischemia. The dose of TSPN (75 mg/kg) was suspended in 0.9% saline (10 g/L), once per day for 1, 3, 7, 14 days after reperfusion. While rats in the vehicle group were treated with equal volume of 0.9% saline, one injection per day until the rats were sacrificed at either 1, 3, 7, and 14 days after brain ischemia. The BrdU and Doublecortin (DCX) expression in SVZ was assessed by immunohistochemistry and applying the immunofluorescence double-labelling to detect the BrdU/DCX, DCX/Ki67, and GFAP/DCX in SVZ. Results: In comparison with the vehicle group, the number of BrdU+ cells in SVZ of TSPN group was significantly higher on days 7 and 14 (P < 0.01, 0.001); Statistical meaning existed in two groups on days 7 and 14 about the mean optical density of DCX+ cells in SVZ (P < 0.01, 0.001). In comparison with the vehicle group, the number of the BrdU-labeled cells co-expressing DCX in the SVZ on day 14t of TSPN group was significantly different (P < 0.01, 0.001). There was statistical meaning in comparison of the number of colocalization of DCX with Ki67 on days 7 and 14 in SVZ between the TSPN group and vehicle group (P < 0.01, 0.001). The ratio of GFAP/DCX to DCX in SVZ of two groups were statistically different on days 2, 7, and 14 (P < 0.05, 0.001). Conclusion: TSPN could promote the neuroregeneration, drive the proliferation and differentiation of neural progenitor cells, and enhance the differentiation of gliosis into newborn immature neurons in SVZ of rats with global cerebral ischemia.