Isolation and identification of fungal endophytes from Ophiopogon japonicus and their antimicrobial activities / 中草药

ABSTRACT

Objective: To explore the resource diversity and specificity of fungal endophytes from the Ophiopogon japonicas and their antimicrobial activities. Methods: Fungal endophytes were isolated from roots and leaves of wild and cultivated O. japonicas by tissue block method. Those fungal endophytes were identified by morphological methods and phylogenetic analysis of ITS sequences. And the antimicrobial activities of those fungal endophytes were estimated by agar disk containing mycelium method. Results: Twenty-seven fungal endophytes strains were isolated from O. japonicas. There was more fungal endophytes diversity in wild plants (22 strains) than cultivated plants (5 strains). Likewise, there was more fungal endophytes diversity in roots of O. japonicas (17 strains) than the leaves (10 strains). And Fusarium and Aspergillus were the dominant genus in O. japonicas. The F. spp. fungal endophytes only isolated from roots while A. spp. from both roots and leaves. In addition, 40% fungal endophytes displayed potent inhibition of Moniliaalbican. Only one or two fungal endophytes strains showed obvious inhibition of Staphyloccocus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis. Conclusion: O. japonicas is rich in fungal endophytes and those fungi distributed with relative specificity characteristics on environment and space. Those fungal endophytes display the antimicrobial activities, especially stain H5, 031p, and H8 with potent inhibition activity are worthy of further research, development, and application.