Preparation of paeoniflorin lipid liquid crystalline nanoparticles and its in vitro release / 中草药

ABSTRACT

Objective: To prepare paeoniflorin lipid liquid crystalline nanoparticles (Pae-LLCN), and to study its in vitro release behavior. Methods: Using encapsulation efficiency (EE) as index, the Pae-LLCN were prepared by spontaneous emulsification and ultrasonic method, and the prescription of Pae-LLCN was optimized by orthogonal design. The in vitro release of Pae-LLCN within 24 h was measured by dialysis method, afterwards its morphology and particle size were studied by transmission electron microscope (TEM). Results: The optimal formulation was poloxamer-glycerol monooleate (1∶10), paeoniflorin inventory (40 mg), and PBS solution (20 mL). The average EE was 73.72%, the average DL was 14.81%, the size of nanoparticles was (170 ± 16) nm, and the 24 h in vitro accumulative release rate was 72.68%. Conclusion: The optimized process is rational and feasible, and the Pae-LLCN has good stability with better sustained release in vitro.