Exploration of in vitro channel tropism research methods of Chinese materia medica by supramolecular imprinting function of alizarin / 中草药

ABSTRACT

Objective: Based on supramolecular imprinting template self-recognition theory, to lay the foundation for the in vitro channel tropism research methods of Chinese materia medica (CMM). Using alizarin as imprinting template feature monomer, in vitro affinity chromatography of different pig organs was performed to test the mechanism of alizarin belonging to the liver meridian. Methods: The liver, lungs, tongue, abdominal muscle, leg muscle groups, and other tissues of pigs, as stationary phases, were made into column chromatography. Using PBS as the mobile phase, in vitro affinity chromatography experiments with alizarin belonging to the liver meridian and white sugar belonging to the spleen meridian were conducted, the content of eluent alizarin was measured by HPLC and the content of sugar was measured by UV. By using the analysis of total statistical moment, the various chromatographic parameters and chromatography equations were obtained. Results: The chromatographic parameters for the alizarin in liver, lungs, tongue, abdominal muscles, leg muscles were as following C0 0.2509-4.813 mg/mL; AUC 2.509-48.13 mg; VR 20.42-30.77 mL; σ2 282.6-532.7 mL2; H 14.13-26.63 mL; n 1.212-1.777; S 0.759 0-1.000. In the positive control group, the adsorption capacity of liver for alizarin was the strongest, and abdominal, leg, lungs, and tongue were weakened in turn. While in the negative control group, the adsorption capacity of liver for glucose (white sugar) was not the strongest. Conclusion: The adsorption capacity of liver for alizarin is the strongest, and liver has an affinity for alizarin by the super molecular imprinting templates, and it matches the theory of meridian belonging to the liver, which would establish the in vitro channel tropism preliminary research methods of CMM.