Comparison on different extraction processes of total flavonoids in Chrysanthemum indicum and its anti-oxidant effect / 中草药

ABSTRACT

Objective: To study three different extraction processes of the content of total flavonoids in Chrysanthemum indicum and their anti-oxidant activities. Methods: The reflux, ultrasonic, and tissue-breaking methods were used for extracting the total flavonoids from C. indicum, which were determined by UV spectrophotometry, and the anti-oxidant activity was determined by flow injection chemiluminescence. Results: The contents of total flavonoids obtained using various methods, such as reflux, ultrasonic, and tissue- breaking, were 12.60, 11.02, and 10.95 mg/g, and their IC50 were 2.67, 3.43, and 5.13 μg/mL, respectively. Conclusion: The research indicates that the reflux extraction method is applied to isolatting the total flavonoids from C. indicum, with the advantage of high extraction efficiency, and the total flavoniods show the strong anti-oxidant activity.