Research on analysis method for chemical variability of E'jiao based on 1H-NMR metabolomics / 中草药

ABSTRACT

Objective: The variability of the acid hydrolysis ingredients of E'jiao from different manufacturers was analyzed with the 1H-NMR-based metabolomics approach. Methods: E'jiao from five different manufacturers was hydrolyzed using acid and then analyzed using 1H-NMR. The component belongings were recognized and analyzed combined with relevant software for the multivariate statistical analysis, and then difference components were figured out. Results: Seventeen substances were recognized in the 1H-NMR profiling. After comparing E'jiao from manufacturers A, C, D, and E with that from manufacturer B respectively, the difference components including isoleucine, hydroxyproline, arginine acid, leucine, phenylalanine, valine, lysine, and levulinate were confirmed. Conclusion: 1H-NMR-based metabolomic study on E'jiao from different manufacturers could be used to analyze the difference components, and provide the new way and reasonable idea for quality control study of E'jiao.