Simultaneous determination of nine components in Zhenlong Xingnao Capsule by UPLC-DAD / 中草药

ABSTRACT

Objective: To develop an UPLC-DAD method for simultaneous determination of gallic acid, cinnamic acid, licorice glycosides, crocin I, crocin II, cinnamic aldehyde, eugenol, glycyrrhetinic acid, and muscone in Zhenlong Xingnao Capsule. Methods: Analysis was performed on a RP-UPLC method and an Agilent Zorbax C18 chromatographic column (100 mm × 2.1 mm, 1.8 μm) eluted with 0.1% formic acid (mobile phase A) and acetonitrile (mobile phase B), the flow rate was 0.3 mL/min, gradient elution and column temperature was 35℃, the injection volume was 1 uL. Respectively to measure their extraction solvent, linear range, precision, repeatability, stability, and recovery rate. Results: The nine components were well separated and showed good linearity, such as gallic acid 0.2-20.0 mg/L (r = 0.999 0), crocin I 0.022-2.200 mg/L (r = 0.999 3), crocin II 0.02-2.00 mg/L (r = 0.999 6), licorice glycosides 0.093-1.860 mg/L (r = 0.999 4), cinnamic acid 0.020 4-2.040 0 mg/L (r = 0.999 9), cinnamic aldehyde 0.042-4.200 mg/L (r = 0.999 9), eugenol 0.95-95.00 mg/L (r = 0.999 9), glycyrrhetinic acid 0.012 9-1.290 0 mg/L (r = 0.998 9), and muscone 0.073 8-7.380 0 mg/L (r = 0.999 0). The precision was good, RSD was 1.02% or less, The repeatability is good in terms of RSD of 1.13% or less and the recovery rate was 93.8%-112.1% (RSD 1.28% or less). Test solution was stable at room temperature within 24 h. The contents of three batches of the gallic acid, crocin I, crocin II, licorice glycosides, cinnamic acid, cinnamic aldehyde, eugenol, glycyrrhetinic acid, and muscone were 0.422-0.448, 0.093-0.105, 0.096-0.112, 0.026 8-0.028 5, 0.142-0.153, 0.140-0.158, 1.519-1.547, 0.007 55-0.008 04, 0.117-0.121 mg/g, respectively. Conclusion: The method is rapid and has high sensitivity, high accuracy, and good specificity, It can be applied to the quality control of Zhenlong Xingnao Capsule.