Protection of Danshen Formula Granula against damage of PC12 cells induced by alcohol / 中草药

ABSTRACT

Objective: To investigate the protective effects of Danshen Formula Granula (DFG) against the damage of pheochromocytoma (PC12) cells induced by alcohol. Methods: PC12 cells were treated with alcohol and DFG at different concentration. The survival rates of PC12 cells were measured by MTT method and the protective effects of DFG against the damage of PC12 cells induced by alcohol were evaluated using the lactate dehydrogenase (LDH) leakage assays (P < 0.05). The apoptosis rates in PC12 cells were examined by TUNEL and Annexin V/PI label staining (P < 0.05). The antioxidant mechanism was assayed by detecting the activities of total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxide enzyme (GSH-Px), and the levels of malondialdehyde (MDA) and intracellular reactive oxygen species (ROS) (P < 0.05) were detected by DCFH-DA staining method. Results: DFG could reduce the damage of PC12 cells induced by alcohol and significantly decrease the apoptosis rate induced by alcoholic damage in PC12 cells (P < 0.05). Meanwhile, DFG could increase the activity of T-SOD, GSH-Px, while CAT, the amounts of MDA, and intracellular ROS were decreased. Conclusion: DFG has the effective protection against the damaged PC12 cells induced by alcohol. The mechanism may be involved in increasing the cell anti-oxidative function.