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Purification technology of Lonicerae Japonicae Flos materiomic based on its fingerprint / 中草药
Chinese Traditional and Herbal Drugs ; (24): 55-60, 2015.
Artículo en Chino | WPRIM | ID: wpr-854156
ABSTRACT

Objective:

To optimize the technological parameters in the purification process of Lonicerae Japonicae Flos (LJF) materiomic with HPLC fingerprint technique.

Methods:

Using the established HPLC fingerprint of LJF, the macroporous resin type, adsorption conditions, and elution requirements were optimized using the similarity and recovery rate of fingerprints of eight components (neochlorogenic acid, chlorogenic acid, cryptochlorogenin acid, vogeloside, luteoloside, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C) as indexes.

Results:

XDA-8 type resin possessed the highest recovery rates of eight components. The optimized purification process was as follows The sample concentration was 0.25 g/mL, the ratio of sample to resin was 0.9 g/g, pH value was 3.5, and 40% ethanol (four times of resin volume) and 80% ethanol (four times of resin volume) were used as eluant, respectively. After being purified, the recovery rates of eight components were 78%-96%, and the similarity of fingerprint was higher than 0.98. These components could get balanced recovery.

Conclusion:

Based on HPLC fingerprint technique, the optimized purification process of LJF materiomic with macroporous resin can ensure that the main components are consistent with the crude extract of LJF.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Traditional and Herbal Drugs Año: 2015 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Traditional and Herbal Drugs Año: 2015 Tipo del documento: Artículo