Chemical constituents from leaves of Ananas comosus and their biological activities / 中草药

ABSTRACT

Objective: To investigate the chemical constituents from the leaves of Ananas comosus and their biological activities. Methods: The chemical constituents from the air-dried leaves of A. comosus were isolated and purified by the chromatography on silica gel and Sephadex LH-20 columns as well as recrystallization. Their structures were identified on the basis of physiochemical properties and spectroscopic data analyses, and the antibacterial activity and artemia lethal activity of the compounds were determined. Results: Eight compounds were isolated from the 95% ethanol extract in the leaves of A. comosus and identified as tricin-4′-O-[10″-O-(8″-hydroxyl) feruloyl-(9‴-O-p-coumaroyl) glyceryl] ether (1), 2, 4-dichlorobenzoic acid (2), tricin (3), chrysoeriol (4), 1-O-p-coumaroylglycerol (5), 1-O-feruloylglycerol (6), 1-O-feruloyl-3-O-p-coumaroyl-glycerol (7), and 1, 3-O-diferuloylglycerol (8). Compound 1 exhibited as well inhibitory activities as positive control Ciprofloxacin (CPFX) against Staphylococcus aureus and Escherichia coli, with MIC values of 0.156 μg/mL. The results of artemia lethal activity showed that the IC50 of the compounds 1 and 4 were 21.4 and 25.0 μg/mL, respectively. Conclusion: All the compounds except 5 are isolated from this plant for the first time. Among them, compound 1 is a new one and named ananasin A, which has the good antibacterial and artemia lethal activity.