Rapid determination of 34 chemicals illegally added into Chinese patent medicines and health foods with blood pressure lowering function by UPLC-MS/MS / 中草药

ABSTRACT

To establish a rapid and accurate method for the determination of 34 chemical hypotensors which were illegally added into the blood pressure lowering class Chinese patent medicines (CPM) and the blood pressure regulating class health foods. The UPLC-MS/MS method was adopted. The samples were extracted with methanol by ultrasonic processing and separated on a Waters Acquity BEH-C18 (100 mm × 2.1 mm, 1.7 μm) column with 0.1% formic acid methanol (A) and 0.1% formic acid water solution (B) as the mobile phase by gradient elution (0-5 min, 32% A; 5-8 min, 32%-50% A; 8-12 min, 50% A; 12-14 min, 50%-60% A; 14-16 min, 60%-80% A; 16-18 min, 80% A; 18-19 min, 80%-90% A; 19-20 min, 90%-100% A; 20-21 min, 100% A; and 21-22 min, 100%-32% A) at a flow rate of 0.2 mL/min, and the column temperature was 40℃. A positive-ion (ESI+) source and a MRM mode were used to separate and quantitatively determine the chemical hypotensors. The obtained molecular ions, fragment ions, and retention time for MRM channels were used to identify the 34 kinds of drugs by comparison with those of reference substances. The obtained peak areas were used to determine the accurate content of chemical hypotensors in the blood pressure lowering class CPM and the blood pressure regulating class health foods. A good resolution of 34 kinds of chemical drugs, including clonidine, captopril, yohimban-16-carboxylicacid, L-tyrosine, hydrochlorothiazide, furosemide, indapamide, minoxidil, hydralazine, atenolol, lisinopril, dibazole, metoprolol, bisoprolol, prazosin, terazosin, propranolol, enalapril, quinapril, benazepril, dilthiazem, doxazosin, nicardipine, nifedipine, amlodipine, nimodipine, felodipine, nitrendipine nisoldipine, valsartan, telmisartan, candesartan cilexetil, rbesartan, and olmesartan medoxomil was obtained under this UPLC and MS/MS condition. The limits of qualitative and quantitative were in the range of 0.1-0.5 and 0.3-1.5 ng/g. The standard addition recoveries were in the range of 81.4%-118.9%. The method is simple, accurate, and with high sensitivity, which can be used for the determination of illegally added chemical hypotensors in CPM and health foods.