Genetic diversity analysis on Ephedra intermedia from Gansu based on ISSR / 中草药

ABSTRACT

Objective: To investigate the genetic diversity of Ephedra intermedia from different populations in Gansu. Methods: ISSR markers were used to analyze the genetic diversity of 11 populations of E. intermedia. Nei's genetic diversity index (H) and other parameters of genetic information were calculated by POPGEN 32. UPGMA relationship dendrogram was clustered by NTSYS. Results: Twelve primers produced 185 bands, among which 150 were polymorphic bands, and the percentage of polymorphic loci (PPL) was 85.71%. H and Shannon's information index (I) were 0.211 4 and 0.332 1, and genetic differentiation coefficient (Gst) and gene flow (Nm) were 0.259 3 and 1.428 6 within the population levels. The genetic distance varied from 0.014 4 to 0.159 8. Conclusion: The genetic diversity among the populations of E. intermedia is at higher level, but the level of genetic diversity within populations than between populations. Genetic variance of E. intermedia mainly exists among populations. The genetic distance of E. intermedia is associated with geographical distance.