Preparation and properties of nepeta oil-oxymatrine lipidosome pro vagina thermosensitive gel / 中草药

ABSTRACT

Objective: To prepare the nepeta oil-oxymatrine (OMT) lipidosome pro vagina thermosensitive gel, and to investigate its in vitro drug release behavior. Methods: P-407 and P-188 were used as gel matrix to prepare the gel, and gelatinization temperature was applied as a target to optimize the prescription. The OMT lipidosome was prepared based on the multiple emulsion method, and the nepeta oil-OMT lipidosome pro vagina thermosensitive gel was obtained by cold-dissolving method. The content of OMT was determined by HPLC, and in vitro release properties of nepeta oil-OMT lipidosome thermosensitive in situ gel was investigated by dialysis method. Results: After optimization, the gel prescription was finally confirmed as 18% P-407, 5% P-188, and 0.2% hydroxy-propyl methyl cellulose (HPMC). The gelatination temperature for nepeta oil-OMT lipidosome thermosensitive gel was (36.8 ± 0.2)°C, and the in vitro accumulating release ratio of sinomenine in the nepeta oil-OMT lipidosome gel system was (58.89 ± 0.34)% and (66.38 ± 0.12)% after 48 h. Conclusion: The prepared nepeta oil-OMT lipidosome thermosensitive gel has the temperature sensitivity and sustained release effect, can effectively delay the release of the drug in vagina and improve the residence time in the vagina.