Protection of Danqi Piantan Capsule on experimental cerebral ischemia-reperfusion injury model in rats / 中草药

ABSTRACT

Objective: To study the protective effect of Danqi Piantan Capsule (DPC, a capsule made of Salviae Miltiorrhizae Radix, Astragali Radix, Paeoniae Rugra Radix, etc. for the poststroke recovery) on the cerebral ischemia in the rat model of cerebral ischemia-reperfusion and its mechanism. Methods: One hundred and eighty male SD rats were randomly divided into six groups: Sham operation, model, positive drug (6.58 mg/kg), the high-, mid-, and low-dose (306, 153, and 76 mg/kg) DPC groups. The rats were ig administered once daily for consecutive 3 d, and the rat models with left middle cerebral artery occlusion (MCAO) were established at 1 h after the last administration. The blood samples were collected from the fossa orbitalis vein of rats at 24 h after the surgery, used to determine the content of serum superoxide dismutases (SOD) and endothelin (ET). The brain tissue samples were collected at 72 h after the surgery for determining the cerebral infarct size and cerebral index through TTC-staining. Results: Compared with the model group, the high- and mid-dose DPC could significantly reduce (P < 0.05) the cerebral index for the cerebral ischemia-reperfusion injury of rats; The three groups of DPJ all could enhance the activity of SOD, especially the high- and mid-dose groups had the significant difference (P < 0.05); The three groups also could reduce the content of ET, but there was no significant difference. Conclusion: DPC has the protective effect on cerebral ischemia-reperfusion and its mechanism may be related with relieving cerebral edema, anti-oxidantion, and inhibiting ET.