Preparation of arsenic trioxide liposome by active drug-loading method / 中草药

ABSTRACT

Objective: To prepare arsenic trioxide (As2O3) liposome, to optimize the formulation of As2O3 liposomes, and to study the feasibility of As2O3 liposomes prepared by the active drug-loading. Methods: Liposomes were prepared by the active drug-loading method. An orthogonal test was utilized to optimize the formulation and preparation of As2O3 liposomes. The unencapsulated As2O3 and liposomes were separated by Sephadex gel G-50 column, the entrapment efficiency (EE) was detected by atomic fluorescence spectrophotometry. The morphological examination of As2O3 liposomes was performed using transmission electron microscopy. The particle size and Zeta potential of the liposomes were measured. The advantage of As2O3 liposomes made by active drug-loading method was investigated. Results: The EE of the liposomes was (72.3 ± 0.8)%, the morphology of liposomes was spherical or ellipsoidal shape with uniform particle size, the mean partical size was (193 ± 12) nm, and Zeta potential was (36.1 ± 3.0) mV. Liposomes have good safety and better stability. Conclusion: The selected formulation and preparation technique of As2O3 liposomes could be rational and stable, and the prepared As2O3 liposomes have good safety.