Effect of extract from Trichosathis Fructus, Carthami Flos, Chuanxiong Rhizoma, and Chrysanthemi Flos on oxidative stress-induced gut immunity in Drosophila melanogaster / 中草药

ABSTRACT

Objective: To study the effect of the extract from Chinese materia medica (CMM) on oxidative stress-induced gut immunity of Drosophila melanogaster. Methods: D. melanogaster was treated by Paraquat and H2O2 with or without CMM, and the effects of 50 kinds of CMM on the survival rate of D. melanogaster in response to oxidative stress were recorded. And the antioxidant effects of CMM on lifespan and the number of ISC and enteroblast (EB) in intestinal epithelium cells were analyzed. Results: The extracts from Trichosanthes kirilowii (Trichosathis Fructus), Carthamus tinctorius (Carthami Flos), Ligusticum chuanxiong (Chuanxiong Rhizoma), and Chrysanthemum monfolium (Chrysanthemi Flos) could significantly improve the survival rate of Paraquat and H2O2-induced D. melanogaster, increase the lifespan of D. melanogaster, and regulate Paraquat-induced and age-related changes in the number of ISC and EB. Conclusion: The extracts from T. kirilowii, C. tinctorius, L. chuanxiong, and C. monfolium could significantly improve the oxidative stress-induced gut immunity of D. melanogaster.