Component analysis of crude and sweated Dipsaci Radix based on HPLC-ESI-MS / 中草药

ABSTRACT

Objective: A high performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS) method was developed for the component analysis of the crude and sweated Dipsaci Radix. Methods: Analyses were carried out on an Agilent Eclipse XDB-C18 (250 mm × 4.6 mm, 5.0 μm) column by gradient elution using (A) 0.1% aqueous formic acid and (B) acetonitrile. The column was maintained at 30℃, and the flow rate was 0.8 mL/min. Chemical characteristics of the crude and sweated Dipsaci Radix were analyzed by MS techniques with an ESI source, the quasi molecular ions of compounds in both negative and positive modes were observed for molecule mass information as [M-H]-, [M + HCOO]-, [M + H]+, and [M + Na]+. Results: An HPLC-ESI-MS spectrum of the crude and sweated Dipsaci Radix was established with 42 peaks respectively, and the potential structures of 31 characteristic components were identified by study on the MS of compounds and comparing with reference data and some of standards. After being sweated, the contents of some components in Dipsaci Radix decreased, such as loganic acid, 4-O-caffeoylquinic acid, chlorogenic acid, caffeic acid, loganin, isochlorogenic acid B, dipsanoside A, and dipsanoside B, but some ones increased, such as cantleyoside, dipsacoside X, dipsacoside VI, dipsacoside VII, dipsacoside B, dipsacoside XIII, and dipsacus saponin A, The amount of other ingredients are almost the same. Conclusion: The HPLC- ESI-MS spectrum could comprehensively control the quality of the crude and sweated Dipsaci Radix.