Protection of extract from Phyllanthus emblica on focal cerebral ischemia-reperfusion injury in rats and its mechanism / 中草药

ABSTRACT

Objective: To study the protection of extract from Phyllanthus emblica (EPE) on focal cerebral ischemia-reperfusion injury in rats and its mechanism. Methods: Male SD rats were divided into six groups such as Sham, model, Ginkgo biloba extract (100 mg/kg, positive control), low-, mid-, and high-dose (crude drug 6.0, 3.0, and 1.5 g/kg) EPE groups. The rats in the treatment groups were ig administered once daily for 10 d. On day 10 the focal cerebral ischemia-reperfusion rat model was established using middle cerebral artery occlusion method. After the model establishment, the neurological function scores were observed, the infarct size was measured by TTC staining, and the contents of SOD, MDA, NO, IL-1β, IL-6, iNOS, and NF-κB in brain tissue were measured. Results: Compared with the model group, EPE could significantly reduce the neurological function scores (P < 0.05), decrease the cerebral infarct size (P < 0.05), increase the activity of SOD (P < 0.05), and reduce the contents of MDA, NO, IL-1β, IL-6, iNOS, and NF-κB (P < 0.05) in brain tissue. Conclusion: EPE has the significant protection on cerebral ischemia-reperfusion injury in rats due to increasing the anti-oxidant activity and inhibiting the inflammatory reaction.