Correlation between absorption ingredients in Wuzhuyu Decoction and uptake amount in everted intestinal sac / 中草药

ABSTRACT

Objective: To explore the influence of the contents of nine ingredients in Wuzhuyu decoction on its own uptake amount into the everted intestinal sac, in order to blend Wuzhuyu decoction samples to improve the index of migraine model rats. Methods: The everted intestinal sac model was established to determine the absorption samples of 10 kinds of Wuzhuyu decoction. HPLC-DAD method was used to analyze the contents of nine ingredients in Wuzhuyu decoction and absorption samples quantitatively and semi-quantitatively. The partial least squares regression method was used to correlate the contents in the decoction and the total absorption quantity of nine ingredients. Results: In the experimental concentration range, the higher the concentration of limocitrin-3-O-β-D-glucoside, ginsenoside Rg1, 6-gingerol, and ruteavin in the decoction were, the more they were absorbed into the intestine sac, while ginsenosides Re, Rb1, limonin, evodiamine, and rutaecarpine were contrary. The interaction of different ingredients on each other's absorption was complicated. Conclusion: The above relationship should be considered to obtain the satisfactory samples of Wuzhuyu decoction.