Effect of different compatibility of Sargassum and Glycyrrhizae Radix in Haizao Yuhu Decoction on goiter model rats and its mechanism / 中草药

ABSTRACT

Objective: To explore the mechanism and compensation proceeding of Haizao Yuhu Decoction (HYD) with different compatibility of Sargassum and Glycyrrhizae Radix in goiter model rats caused by propylthiouracil (PTU). Methods: HYD with different compatibility of Sargassum and Glycyrrhizae Radix was set up according to the principle uniform design (two factors, seven levels), and 120 Wistar rats were randomly divided into 10 groups. PTU was ig given to rats for 14 d to establish the goiter model, and then the rats were ig administered with HYD for 28 d. All animals were sacrificed after the last dosage, the thyroid weight and changes of thyroid hormone levels of triiodothyronine (T3), thyroxine (T4), and thyroid stimulating hormone (TSH) in serum were determined, and the mRNA expression of thyroid peroxidase (TPO) and thyroglobulin (Tg) was detected by RT-PCR. Results: HYD with different compatibility of Sargassum and Glycyrrhizae Radix could regulate thyroid hormone levels and inhibit thyroid enlargement induced by PTU in some degree. Compared with the model group, the TPO mRNA expression of group 5 (0.135:8.32) was significantly increased, and groups 1 (14.522:6.24), 5, and 6 (29.040:4.16) could also increase the Tg mRNA level significantly. Conclusion: HYD with different compatibility could inhibit thyroid enlargement induced by PTU in some extent, the effects in groups 1 (14.522:6.24), 3 (19.363:0.54), and 6 (29.040:4.16) are the most obvious, and the mechanism may be related to the activation of gene transcription of TPO and Tg. The groups 1 and 5 could enhance the compensatory action to a certain degree.