ISSR analysis and identification on Isatis indigotica from different habitats / 中草药

ABSTRACT

Objective: To study the genetic diversity and genetic relationship of Istais indigotica from different habitas by ISSR-PCR marker technique, so as to provide the reference for its germplasm identification and breeding. Methods: Twenty-three samples of I. indigotica and its variation types from the different habitas of China were studied by ISSR-PCR markers. The Jaccard coefficient was worked out by NTSYS-pc software, and a cluster dendrogram of different samples was established based on the unweighted pair-group method with arithmetic mean (UPGMA). Results: Ten ISSR primers generated 109 loci of which 88 loci were polymorphic. The average percentage of polymorphic bands (PPB) was 80.7%. The germplasm of I. indigotica had the plentiful genetic diversity. In the cluster dendrogram, the studied samples were clustered into three groups, the first one included majority samples, the second was the variation type with hair, and the third one was the farthermost, which was separated at the coefficient of 0.62. Conclusion: ISSR method is suitable for the germplasm identification and genetic diversity analysis of I. indigotica, thus providing a biological theoretical foundation for its cultivation and breeding.