Isolation and identification for strains from wild Phellinus and analysis on its secondary metabolites / 中草药

ABSTRACT

Objective: To isolate and identify the strains from wild Phellinus linteus and analyze its secondary metabolites. Methods: Strains isolated and purified were investigated by optical microscope to observe the characteristics of mycelia, ITS sequence was used for molecular identification and its secondary metabolite content was analyzed by chemical colorimetry. Results: The morphological characteristics of mycelia from pinus, Morus alba, Syringa reticulata, and populus were the same. The growth rate of four flamentous colonies and their colors were different, wherein the growth rate of the colony from M. alba was the fastest, up to 0.47 cm/d. Four strains all belonged to genus Phellinus sp, where strain from pinus was identified as P. pini, M. alba was P. linteus, S. reticulata and populus were P. baumii. The species of the secondary metabolites in the sporocarp and mycelia of Phellinus were the same, but the content was different. For the sporocarp, the highest polysaccharide content was in the sporocarp from S. reticulata with the content of 98.20 mg/g, the highest flavonoids, terpenoids, and polyphenols contents were all in the sporocarp from poplar with the contents of 548.49, 1.48, and 33.70 mg/g, respectively. For mycelia, the highest polysaccharide, flavonoids, terpenoids, and polyphenols contents were all in the mycelia of M. alba, the contents were 259.64, 223.11, 43.78, and 24.80 mg/L, respectively. Additionally, the content of terpenoids in the mycelia was higher than that of the sporocarp. Among them, the content of terpenoids in the mycelia from M. alba was about 7 times as that in fruit body. Conclusion: There are no differerences amony form of mycelia from four strains of P. linteus. rDNA ITS sequence analysis could be used for the identification of strains. The species four Phellinus strains and its secondary metabolite content are clarified.