Chemical constituents in antiviral fraction of Isatidis Radix and their activities / 中草药

ABSTRACT

Objective: To study the chemical constituents in the antiviral fraction of Isatidis Radix and the activity of the monomer components. Methods: The water extract of Isatidis Radix was adsorbed by macroporous resin, and the antiviral fraction was eluted with 10% and 50% ethanol, repeatedly separated and purificated by silica gel column chromatography, reverse phase silica gel column chromatography, and Sephadex LH-20. The chemical structures were identificated using physicochemical constants and spectral data. Results: Ten compounds were isolated from the active fraction of water extract, and they were respectively identified as syringin (1), 4-(1, 2, 3-trihydroxypropyl)-2, 6-dimethoxyphenyl-1-O-β-D-glucopyranoside (2), isolariciresino (3), isolariciresinol 4-O-β-D-glucopyranoside (4), lariciresinol-4-O-β-D-glucopyranoside (5), lariciresinol-4, 4'-bis-O-β-D-glucopyranoside (6), 2-hydroxy-1, 4-benzenedi carboxylic acid (7), D-mannitol (8), indole-3-acetonitrile6-O-β-D-glucopyranoside (9), and 3-[2'-(5'-hydroxymethyl) furyl]-1(2H)-isoquinoline-7-O-β-D-glucopyranoside (10). Conclusion: Compounds 2, 4, 7, 8, and 10 are isolated from Isatidis Radix for the first time. Compound 10 has the significant effect of anti-HSV-2 virus. Therapeutic index (TI) is 6.07. Compounds 1, 5, and 6 also show some inhibition to HSV virus.