Purification of total flavonoids from diaphragma of Juglans regia by macroporous resin / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2688-2692, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-855110
ABSTRACT
Objective:
To investigate the purification technology of total flavonoids from the diaphragma of Juglans regia by macroporous resin.Methods:
Twelve macroporous resins were chosn with static and dynamic adsorption and desorption experiments to optimize the purification parameters.Results:
AB-8 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were pH value of 4.70, sample mass concentration of 0.713 5 mg/mL, the loaded amount of 4 mL/g, and loading flow rate of 1.5 BV/h. The sample was eluted by water with 2 BV and 50% ethanol of 2.5 BV, respectively. The purity of total flavonoids increased to 72.25% after the purification, and the yield was 93.94%.Conclusion:
AB-8 is an ideal resin with the best enrichment for separating and purifying the total flavonoids from diaphragma of J. regia.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Traditional and Herbal Drugs
Año:
2013
Tipo del documento:
Artículo
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