Effect of flavonoid from Glycyrrhizae Radix on small intestine epithelium IEC-6 cell migration and intracellular polyamine content / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2722-2726, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-855116
ABSTRACT
Objective:
To observe the effect of flavonoid from Glycyrrhizae Radix (FGR) on the migration of small intestinal epithelial cells (IEC-6) and intracellular polyamines content.Methods:
The cell migration model was constructed by scratch method. Under the intervention of α-difluoromethylornithine (DFMO), the specific inhibitor of polyamine synthesis, or 4-aminopyridine (4-AP), the specific inhibitor of potassium channel, the effect of FGR on cell migration was observed. The intracellular polyamine content being cultured for 12 or 24 h in normal and DFMO intervention condition was detected by precolumn derivatization-HPLC.Results:
FGR could reverse the inhibitory effects of DFMO and 4-AP on cell migration, increase the intracellular content of polyamines after 12 or 24 h treatment, and reserve the decrease of polyamine content caused by DFMO.Conclusion:
FGR could affect the polyamine-dependent signaling pathway, which may be related to the mechanisms for FGR promoting cell migration.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Traditional and Herbal Drugs
Año:
2013
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS