Separation and purification of phenylethanoid glycosides from Callicarpa kwangtungensis by macroporous resin / 中草药

ABSTRACT

Objective: To study the technology for separation and purification of phenylethanoid glycosides from Callicarpa kwangtungensis by macroporous resin. Methods: The absorption and separation properties of 10 kinds of absorption resins were studied and the separation and purification technological process of phenylethanoid glycosides from C. kwangtungensis were optimized by HPLC with the total content of forsythiaside B and poliumoside as an index. Results: Using X-5 resin column as adsorbent, the optional conditions were as follows loading qualities were 32.79 mg/g, the resin was washed by 4 BV distilled water to remove impurity and 7 BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2 BV/h, and the content of phenylethanoid glycosides in the extract was 52.8% (n = 3). Conclusion: This technology is simple and feasible, and the process with X-5 resin is an effective method to separate and purify phenylethanoid glycosides from C. kwangtungensis.