Analysis on in vivo metabolic pathways of 3-acetyl-11-keto-β-boswellic acid in rats / 中草药

ABSTRACT

Objective: To study the in vivo metabolic pathways of 3-acetyl-11-keto-β-boswellic acid (AKBA) in rats. Methods: An HPLC-QTRAP-MS method was applied to identifying the metabolites of AKBA in bile, urine, and feces after ig administration of AKBA 60 mg/kg to rats. Results: A total of 15 metabolites were found in rats. The major metabolic pathways of AKBA were deacetylation, mono-hydroxylation, dehydrogenation, and glucuronide conjugates. Conclusion: AKBA undergoes the extensive phases I and II metabolism in rats; The metabolites produced mainly excrete through feces and bile; The information-dependent acquisition (IDA) and enhanced product ion (EPI) scan were used on a linear ion trap mass spectrometer to get high quality MS2 picture and provide an effective method for identifying the metabolites of AKBA.