Effect of Xixin Decoction Granule on Tau protein toxicity in brain of rats with sporadic Alzheimer's disease / 中草药

ABSTRACT

Objective: To establish sporadic Alzheimer's disease (SAD) rat model, to investigate the effects of Xixin Decoction Granule (XDG) on the phosphorylation of Thr231 and Ser422 sites as the important promoters of Tau protein toxicity in the brain of SAD rat, and to explore the possible mechanism of XDG on the prevention and treatment of SAD. Methods: The SPF male SD rats were randomly divided into Sham (S), model (M), donepezil (D, positive control), low-, mid-, and high-dose XDG (LX, MX, and HX, 7.61, 15.21, and 30.42 g/kg) groups, with ig administration once daily for two months. The immunohistochemistry and Western blotting were used to detect the phosphorylation levels of Thr231 and Ser422 sites in Tau protein in brain of rats with SAD. Results: Compared with M group, XDG could significantly decrease the expression of Thr231 and Ser422 sites in the hippocampus of SAD rats (P 0.05). Conclusion: The results suggest that XDG could inhibit the hyperphosphorylation of key sites in site protein and Tau toxicity, so as to prevent SAD pathological progress.