Isolation and identification of endophytic fungi from Polygonatum sibiricum in Mountain Tai and study on their antimicrobial activity / 中草药

ABSTRACT

Objective: To study the diversity of endophytic fungi from Polygonatum sibiricum growing in Mountain Tai and screen the endophytic fungi with antimicrobial activity. Methods: Endophytic fungi were isolated with the conventional method and their antimicrobial activities against tested microorganisms were investigated by using the paper disc method. The endophytic fungi with higher antimicrobial activity were identified based on ITS sequence analysis. Results: A total of 32 endophytic fungi strains were isolated from the roots, stems, leaves, and fruits of P. sibiricum belonging to seven genera. The most widely distributed genera is Fusarium sp. (31.3%), followed by Sterile mycelia (28.1%) and Alternaria sp. (21.9%). Some endophytic fungi showed a certain degree of tissue preference. More species and strains were isolated from the roots, followed by those in the stems and leaves but those in the fruits were the least. Fourteen strains from five genera with the antimicrobial activity were obtained, among which eight strains showed broad-spectrum antimicrobial activity. The strain HJG-10 with the highest antimicrobial activity was determined to be Schizophyllum commune, according to its molecular analysis. Conclusion: The research not only provides the theoretical basis for developing and utilizing the resources of endophytic fungi in P. sibiricum in Mountain Tai but also shows a new path for searching novel antimicrobial resource.