Rapid quality control in purifying process of Paeoniae Alba Radix extract using near infrared spectroscopy / 中草药

ABSTRACT

Objective: To establish a fast detection method during the purifying process of the extracts from Paeoniae Alba Radix based on near infrared optical fiber transmission spectroscopy. Methods: The near infrared spectroscopy (NIRS) of the eluates by water and 70% ethanol which flowed through the macroporous resin D-101 was measured by NIR transmission optical fiber probe, and the method of pattern recognition was used to study the two processes. Aiming at the process of 70% ethanol elution, the contents of constituents were determined by HPLC as the reference values, and partial least square regression (PLSR) was applied to build the calibration models of paeoniflorm and albiflorin. Results: The processes of water elution and 70% ethanol elution could be accurately monitored with pattern recognition, and the changes of the solvent system could be accurately indicated. The quantitative models were applied to successfully predict the elution curves of Paeoniae Alba Radix extracts from the same batch and different batches on macroporous resin. The root mean square errors of prediction (RMSEP) calculated by PLSR models for paeoniflorm and albiflorin were 0.124, 0.172, and 0.120, 0.133, respectively, and the correlation coefficient between the values calculated by NIRS and HPLC was all greater than 0.992. Conclusion: The method is real time, efficient, and fast, and could be applied to the fast analysis for the purifying process of Chinese materia medica.