Effect of Aconiti Cocta Radix combined with Pinelliae Praeparatum Rhizoma in different ratios on Aconitum alkaloids / 中草药

ABSTRACT

Objective: To investigate the change tendency of Aconitum alkaloids in Aconiti Cocta Radix (ACR) combined with Pinelliae Praeparatum Rhizoma (PPR) in different ratios, and to elaborate the chemical material basis of the compatibility of ACR and PPR. Methods: LC-MS technology was used to analyze the decoctions with different ratios after long-time frying, and chemometrics was applied to identify and analyze the chemical markers according to the change of pH value. Results: There were the significant differences in chemical constituents of ACR-PPR compatibility with different ratios. The pH value increases when the ratios of PPR increasing. However, the content of diester diterpene alkaloids was totally decreasing. Conclusion: The toxic substance is decreased when ACR and PPR combined, which could be related to some constituents in PPR affecting the stability of Aconitum alkaloids.