Preparation and process optimization of gambogenic acid inclusion complex of freeze-dried powder injection / 中草药

ABSTRACT

Objective To optimize the preparation of gambogenic acid hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex of freeze-dried powder injection. Methods Gambogenic acid HP-β-CD inclusion complex of freeze-dried powder injection was prepared by freeze-drying method, gambogenic acid content was determined by HPLC, and the preparation was optimized by orthogonal design taking inclusion rate as index. Results The optimized preparation process for inclusion was that the reaction mass charge ratio (HP-β-CD-gambogenic acid) was 10 : 1, for 5 h at 20°C. The inclusion rate of prepared inclusion complex was higher; The freeze-dried powder was with color uniformity, fluffy texture, and good solubility. Conclusion The process is reasonable and with good reproducibility.