Antitumor effect of Macleaya cordata and its molecular mechanism on inducement of human telomeric DNA to form G-quadruplex / 中草药

ABSTRACT

Objective: To study the antitumor molecular mechanism started on sanguinarine (San) and chelerythrine (Che) from Macleaya cordata. Methods: Determining the IC50 values of San and Che against A-549, HCT-8, and Bel-7402 cell lines by using MTT to clarify whether these two alkaloids are the active components in M. cordata; studying the interaction between human telomeric DNA and two alkaloids respectively by using UV-Vis, FL, and CD methods. Results: San and Che are the active antitumor components of M. cordata; San could induce HT4 to form antiparallel G-quadruplex completely with Ka of 5×108 and Che could induce HT4 to form antiparallel G-quadruplex partially with Ka of 930. Conclusion: One of the antitumor molecular mechanisms of M. cordata is that the two active components could induce human telomeric DNA to form G-quadruplex.