Analysis of genetic diversity for wolfberry germplasms by AFLP technology / 中草药

ABSTRACT

Objective: To study the genetic relationships of fifteen wolfberry germplasm, including seven wild species, three varieties, and five cultivars and lines. Methods: Data were analyzed by the technology of genomic DNA-AFLP markers and the NTSYS average similarity cluster analysis. Results: The statistics showed that 432 bands were amplified using eight pairs of primers, polymorphic bands had account of 360 numbers, and polymorphic rate was 83.3%. All germplasm were classified into nine groups, five groups and three groups as different coefficient of 0.72,0.79, and 0.85. Conclusion: This study reveals a diverse basis and their genetic relationship of Chinese wolfberry at the molecular level. The male sterility YX-1 is clustered together with a white flowers wolfberry and a round fruit wolfberry, and YX-1 is more close to the two kinds of wolfberry but farther to the Ningxia wolfberry in the genetic distant. In addition, the classification is obviously different with the traditional morphological one about three variants.