Optimization of extraction process of Yangjing Zhongyu Decoction by orthogonal test / 中草药

ABSTRACT

Objective: To optimize the extraction process of Yangjing Zhongyu Decoction and investigate the content change of loganin, paeoniflorin, and ferulic acid in the mixed and seperated decoctions. Methods: Orthogonal design was used to optimize the extraction process. The contents of the three components were determined under the same chromatographic conditions by HPLC. The detective wavelength of loganin and paeoniflorin was 230 nm, and the detective wavelength of ferulic acid was 316 nm. Results: The optimum extraction condition was as follows six times water, extracting three times, every time for an hour. Conclusion: The optimum process is stable and feasible. The contents of the three components are more improved in the mixed decoction in varying degrees than single decoction.