Effects of He-Ne laser irradiation on bone marrow mesenchymal stem cell growth and neural differentiation potential / 中国脑血管病杂志

ABSTRACT

Objective: To observe the effects of He-Ne laser therapy instrument on bone marrow mesenchymal stem cell (BMSC) growth and neural differentiation potential. Methods: The primary BMSCs from Sprague-Dawley rats were taken using whole bone marrow culture. The passage 3 cells were randomly divided into an experimental group (He-Ne laser therapy instrument treated for 10 min) and a control group (exposing in the air for the same time) according to whether they were treated with the He-Ne laser therapy instrument or not. The cell proliferation activity was measured with cell counting kit-8 (CCK-8) assay. Apoptosis detection was performed using flow cytometry. The cell vitality was detected by Trypan blue staining. Human neural stem cell differentiation potential was identified by immunofluorescence technique. Results: Circled digit oneCells showed a fibroblast-like adherent growth. The phenotypic identification results revealed that the expressions of CD29 and CD90 were positive, but the expression of CD45 was negative, and it is consistent with the characteristics of the BMSC phenotype. Circled digit twoThere was no significant difference in the proliferative activity between the experiment group and the control group, P > 0.05. Circled digit threeThere were no significant differences between the early apoptosis rate (1.10 ± 0.26%) in the experiment group and that (1.43 ± 0.06%) in the control group, as well as the cell viability (96.2 ± 0.6%) in experiment group and that (96.2 ± 0.7%) in the control group. Circled digit fourSimilar to the control group, BMSCs in the experiment group showed the suspended neurospheres after being cultured in neural stem cell culture medium. Immunofluorescence staining showed that the nestin staining of neurosphere cells was positive. Conclusion: He-Ne laser therapy instrument has no significant effects on the proliferation and viability of BMSCs. It does not cause apoptosis and change the potential of BMSCs to neural stem cell differentiation.