A rat model of multiple organ dysfunction syndrome induced by subarachnoid hemorrhage / 中国脑血管病杂志

ABSTRACT

Objective: To establish a rat model of multiple organ dysfunction syndrome (MODS) by subarachnoid hemorrhage (SAH), and to explore the pathogenesis of MODS occured in acute cerebrovascular diseases (ACVD). Methods: Model of SAH was induced in rats by injecting arterial blood around the Willis' circle. 48 Wister rats were randomly divided into three groups: normal control group (n = 6), sham-operative group (n = 6) and SAH group (n = 36). The SAH group was further divided into 6 groups according to the time points of 4h, 12h, 24h, 36h, 48h and 72h after SAH, each group has six rats. Changes of temperature, heart rate and respiratory rate of the rats in each group were recorded, the amount of white blood cell (WBC) and biochemical criteria were assayed. Pathological changes of lung, liver, intestines and kidney were observed under optical microscope. Systemic inflammatory response syndrome (SIRS) and MODS were diagnosed by their criteria. Results: 1 There were no significant differences of temperature, heart rate, respiratory rate and the levels of WBC, ALT, AST, BUN, Cr and CK between normal control group and sham-operative group (P > 0.05). The rats of SAH group had obviously higher temperature, heart rate, respiratory rate, WBC, ALT, AST, BUN, Cr and CK than that of the normal control group and sham-operative group (P < 0.01), the changes were most obvious at 24-36 hour point. 2 The vital organs of the rats showed inflammatory injuries in varying degree at each time point after SAH, and peaked on 24-36 h, alleviated after 48 h, and last to 72 h after SAH. 3 The incidences of SIRS, MODS and mortality were 100% (36/36), 69.4% (25/36) and 38.9% (14/36) respectively in the SAH group. Conclusion: 1 An experimental animal model of MODS can be established successfully by injecting arterial blood around the Willis' circle in rats. 2 SAH can cause inflammatory changes in lung, liver, intestines, kidney as well as SIRS, and SIRS may be the pathological basal of MODS after SAH.