Isochlorogenic acid A inhibits activation of NLRP3/NF-ΚB in rats with collagen-induced arthritis / 中国药理学通报

ABSTRACT

Aim To analyse the anti-inflammatory effect of isochlorogenic acid A in rats with collagen-induced arthritis and the potential mechanism . Methods Male SD rats were randomly divided into normal group, model group, isochlorogenic acid A high and low dose groups. The model was constructed in all groups except control group. After continuous administration for six weeks, the plantar thickness and spleen organ coefficient were measured in rats. NLRP3, caspase-1, NF-ΚB p65, P-NF-ΚB p65, P-IΚB and RANKL protein in synovium of joint were detected by Western blot, and IL-lβ, IL-6, TNF-α, CRP, IFN-γ, IL-18 expressions in blood plasma were detected by ELISA kit. Results Compared with normal group, the swelling degree of plantar and the spleen organ coefficient in model group increased significandy. Western blot showed that the expression of NLRP3, caspase-1, NF-ΚB p65, P-NF-ΚB p65, P-IΚB-O and RANKL protein were up-regulated in synovium of joint tissues in model group. ELISA showed that the expression of blood plasma IL-lβ, IL-6, TNF-α, CRP, IFN-γ and IL-18 were significantly up-regulated in model group. Different doses of isochlorogenic acid A can significantly reduce the performance of the above indicators in model group. Conclusions Isochlorogenic acid A has a good anti-inflammatory effect on collagen-induced arthritis, and its anti-inflammatory activity may be related to the decrease of NLRP3 inflammatory complex activation and NF-ΚB phosphorylation.