Guizhi decoction suppresses mouse allergic contact dermatitis by inhibiting differentiation of lymphocytes to Th2 cells / 中国药理学通报

ABSTRACT

Aim To reveal the effects of Guizhi decoction (G Z D) on allergic contact dermatitis by establishing the mouse allergic contact dermatitis model induced by fluoresciniso thiocyate (FITC) and culturing splenic lymphocytes. Methods BALB/c mice were sensitized by smearing the 1.5% FITC on the shaved abdomens on day 1 and day 2 and elicited on the right ear with 0. 6% FITC solution on day 6. GZD was administered from day 1 to day 7. Ear thickness was measured 24 h after elicitation. HE staining was performed for pathohistological examination. Interleukin-4 (I L - 4), IL-5, IL-9, interferon γ(IFN-γ) in ear tissue homogenates and IgE in serum were detected. The splenic lymphocytes were stimulated with ConA. Results The ear swelling was significantly suppressed by administering GZD. GZD reduced the infiltration of inflammatory cells and edema in the ear, and the levels of I L 4, IL-9, IFN7 and IgE. GZD could also reduce the expression of I L 4, IL-10, IFNγ and GATA3. Conclusions GZD could suppress allergic contact dermatitis elicited by FITC, which may result from inhibiting the differentiation of lymphocytes to Th2 cells.