Pharmacokinetic study on asparaginase-evodiamine loaded core-shell lipidic nanoparticles / 中国药理学通报

ABSTRACT

To investigate the pharmacokinetic behavior of core-shell lipidic nanoparticles (AELNs) loaded with asparaginase and evodiamine in rats, and compare the bioavailability of AELNs with free asparaginase (ASNase)/evodiamine (EVO). Methods Eighteen male Sprague-Dawley rats were randomly divided into three groups and injected with AELNs, free ASNase and free EVO, respectively. After injection, blood samples were taken from the eyelids of rats at different time. The concentration of EVO and ASNase in plasma samples was determined by HPLC and Nessler reagent colorimetry method, respectively. To analyze the differences in pharmacokinetic behavior be-tween AELNs and free ASNase or EVO, their pharmacokinetic parameters were calculated using DAS software. Results and MRTom4thy of ASNase in AELNs were 1. 83 and 1. 94 times higher than those of free ASNase, respectively. The AUC(0-48h) of EVO in AELNs was 29. 94 times that of free EVO. Conclusions Compared with free ASNase and EVO, AELNs have a higher bioavailability in rats.