The protective effect of nerve function of Buyang Huanwu decoction on cerebral ischemia/reperfusion rats after neural stem cell transplantation / 中国药理学通报

ABSTRACT

To explore the protective effect of nerve function of Buyang Huanwu Decoction on cerebral ischemia/reperfusion rats after the transplantation of neural stem cells (NSCs) . Methods Thread bolt method was used to establish middle cerebral artery occlusion model. Drug groups were given Buyang Huanwu Decoction (14. 8 g kg"1 d " 1) by gavage after the rats being sober. NSCs were transplanted to rat brain after making the model 24 hours later. Zea Longa neurobehavioral behavioral score was used to observe neural function defect, and TTC staining to detect the volume of cerebral infarction, and Nissl staining to detect Nissl body integrated optical density (IOD), and Immunohistochemical staining to detect expression of Bcl-2 and Bax. Results Compared with sham operation group, the nerve function defect appeared, and the volume of cerebral infarction increased significantly, the integral optical density of Nissl body was reduced and the ratio of Bcl-2 to Bax was reduced in model group (P < 0. 05) . Compared with model group, the nerve function defect was reduced, the volume of cerebral infarction was reduced, the integral optical density of Nissl body increased, and the ratio of Bcl-2 to Bax increased in BYHWD group, Transplant group and BYHWD + Transplant group (P < 0. 05). Compared with transplant group, the nerve function defect was reduced, the volume of cerebral infarction was reduced , the integrated optical density of Nissl body increased , and the ratio of Bcl-2 to Bax increased in BYHWD + Transplant group (P < 0. 05). Conclusions Buyang Huanwu Decoction can enhance the neuroprotective effect after NSCs transplantation in cerebral ischemia/reperfusion rats.