Paeoniflorin accelerates diabetic wound healing / 中国药理学通报

ABSTRACT

Aim To observe the effectof paeoniflorin (PA) on diabetic wound healing and its mechanisms. Methods A full-thickness skin excision splint model in diabetic mice was used to study the effects of PA on diabetic wound healing and the underlying mechanism. HE staining and Masson staining were used to observe the changes of granulation tissue and collagen in wound tissues. Immunofluorescence technique was employed to detect angiogenesis in wound tissues of diabetic mice. To detect the effects of PA, human umbilical vein endothelial cells ( HUVECs) and mouse fibroblasts were cultured in vitro. MTS, BrdU and scratch assays were used to detect the effects of PA on proliferation and migration. Tube formation assays were used to detect the effects of PA on the tube formation of HUVECs. qPCR was applied to assess the expression of collagen HI, fibronectin and ot-SMA gene. Immunoflu orescence was used to detect the expression of a-SMA. Results Compared with db/db model group, the formation rate of granulation tissues and collagen and the capillary density around the wound of db/db model group treated with PA was significantly higher than that of db/db model group(P <0. 01 ). PA had no significant effect on endothelial cell proliferation, but it could markedly promote endothelial cell migration and tube formation. PA could significantly up-regulate the migration , proliferation, secretion and differentiation of fibroblasts. Conclusions PA can significantly promote diabetic wound healing, which may be related to accelerating the generation of extracellular matrix and promoting angiogenesis.