Protective effect of resveratrol on oxidative stress injury of cardiomyocytes induced by high fat and its correlation with AMPK/mTORC1/p70S6K signaling pathway / 中国药理学通报

ABSTRACT

Aim To investigate the protective effect of resveratrol on oxidative stress injury of cardiomyocytes induced by high fat and its correlation with AMPK/mTC)RCl/p70S6K signaling pathway. Methods The model of cardiomyocyte lipotoxicity injury was established by palmitic acid (PA). Resveratrol pretreatment and MTT assay were used to detect cell proliferation. The expression of reactive oxygen species ( ROS) was detected by fluorescence and flow cytometry, and the levels of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were detected by kit. The protein expression of AMPK/mTORCl/P70S6K signaling pathway and apoptosis related proteins were determined by Western blot. Results MIT results showed that the cell viability decreased significantly at 0. 4 mmol L-1 and 24 h-48 h stimulation time, re-spectively. Fluorescence and flow cytometry showed that the levels of ROS and MDA increased significantly and SOD activity decreased significantly when PA (0. 4 mmol • L"1) stimulated cells for 24 h compared with control group. Western blot showed that the expression of p-AMPK and Bcl-2 decreased and the expression of p-mTORCl, p-p70S6K, Bax and cleaved caspase-3 increased when PA (0.4 mmol • L"1) stimulated cells for 24 h compared with control group. Resveratrol pre-treatment could significantly reverse the above chan-ges. Conclusions Resveratrol can significantly inhibit the apoptosis of cardiomyocytes induced by high fat. Its mechanism may be related to the regulation of AMPK/mTORCl/p70S6K signaling pathway.