Effect of urolithin A on autophagy of myocardial cells in diabetic mice / 中国药理学通报

ABSTRACT

Aim: To explore the effect of urolithin A (UA) on autophagy of cardiomyocytes in diabetic mice. Methods: Glucose (5. 5, 20, 33, 47 mmol · L-1) and UA at different concentrations were used to stimulate the neonatal rat cardiomyocytes for 48 h. MTT assay was used to detect the survival rate of cardiomyocytes. Western blot was used to detect the expression of autophagy proteins LC3 and p62. Healthy male C57BL/6 mice were intraperitoneally injected with STZ for 6 days to establish diabetic mouse model. UA was given by intragastric administration for 10 weeks and the cardiac function indicators were detected by ultrasound. Masson staining was used to observe the myocardial pathological sections. Results: After treatment with 33 mmol · L-1 of high glucose for 48 h, the survival rate of cardiomyocytes decreased, and autophagy protein p62, LC3 II, the ratio of LC3 II/I increased, which were reduced with UA treatment. Compared with the control mice, the cardiac function of diabetic cardiomyopathy mice showed decreased ratio of E/A peak. Masson showed that cardiac fibrosis expression decreased after UA administration. Conclusion UA can protect diabetic cardiomyopathy by affecting myocardial autophagy.