Rhynchophylline reverses methamphetamine-induced CPP by regulating GluRl expression in zebrafish / 中国药理学通报

ABSTRACT

Aim To investigate the effect of rhyncho-phylline on methamphetamine ( METH )-induced condition place preference (CPP) zebrafish and the relationship with GluRl. Methods METH was injected intraperitoneally to zebrafish, and the expression of GluRl in zebrafish brain was detected by immunohisto-chemistry and Western blot. Results METH-induced CPP model was established after the administration of METH (40 mg • kg-1) in zebrafish. Compared to control group, the resident time in white box of model group zebrafish significantly increased; compared to model group, the resident time in white box of ket-amine (150 mg • kg-1) group and rhynchophylline (100 mg • kg-1) group dramatically decreased. Compared to control group, the number of GluRl positive cells and the GluRl expression of model group ze-brafish markedly increased; compared to model group, the number of GluRl positive cells and the GluRl expression of rhynchophylline group zebrafish evidently decreased. Conclusions GluRl is important for METH-induced CPP formation in zebrafish. Further, rhynchophylline reduces GluRl expression in METH-treated zebrafish brain, and its mechanism may be related to GluRl expression in the brain of zebrafish.