Evaluation of hypoxia/reoxygenation injury of H9C2 myocardial cells and intervention effect of notoginsenoside R1 based on RTCA technology / 中国药理学通报

ABSTRACT

Aim: To establish the standard hypoxia/reoxygenation (H/R) injury model of H9c2 myocardial cells and evaluate the intervention effect of notoginsenoside R, based on RTCA technology. Methods: H9c2 myocardial cells were inoculated into the E-Plate board, 3 wells by a group, then the growth curve and cell index of different hypoxia time, different reoxygenation time and notoginsenoside R, intervention were determined respectively, with the growth status of H9c2 myocardial cells reflected by cell index. Results: Cell survival rate was (48.82 ±5.32)% after hypoxia treatment for 4h and reoxygenation treatment for 16h with replacement of serum-free, glucose-free DMEM medium before hypoxia, and no significant difference was found between the results measured with MTT method. Notoginsenoside R, could protect the hypoxia/reoxygenation injury of H9c2 myocardial cells without time dependence. Conclusions: RTCA technology is an effective means to establish the standard H/R injury model of H9c2 myocardial cells, which also has some guiding significance in discovering new drug targets and mechanisms.