The expression changes of BDNF in trigeminal ganglia of rats with trigeminal neuralgia / 中国药理学通报

ABSTRACT

Aim: To observe the expression changes of brain-derived neurotropic factor (BDNF) and receptor TrkB in trigeminal ganglion (TG) of trigeminal neuralgia (TN) rats. Methods: Animal model of TN was established using the infraorbital nerve chronic injury compression model (ION-CCI). The sham operation group (sham group) and the TN model group were composed of randomly divided 180 - 220 g SD male rats. The mechanical threshold of the injury side of the two groups was determined. Quantitative real-time PCR (qPCR), immunohistochemistry and immunfluorescence methods were used to detect the expression of BDNF, TrkB, pro-inflammatory TNF-α and IL-1B in the injured TGs. Results: After two weeks of modeling, the mechanical pain threshold was significantly down-regulated in TN group compared to sham group (P<0. 05). The expression of BDNF and TrkB in TGs of TN group was significantly higher than that in sham operation group. Besides, the levels of pro-inflammatory factors TNF-a and IL-1β also significantly increased in TGs of TN group compared with sham group (P < 0. 05). Conclusions: The expression of BDNF and TrkB in TG of ION-CCI rats increases in trigeminal neuralgia group, which may be involved in the pathogenesis of trigeminal neuralgia and promote the pain transmission of trigeminal neuralgia.