Effects of rhCTLA4Ig on human T lymphocyte in vitro / 中国药理学通报

ABSTRACT

AIM: To study the effect of rhCT-LAIg on human T lymphocytes. METHODS: Primary mixed lymphocyte reaction (MLR), secondary MLR and cytotoxicity assay were used. RESULTS: CTLA4Ig significantly inhibited primary MLR(P<0.05) and the maximal inhibition was achieved at 72 h of incubation with CTLA4Ig. Secondary MLR experiment showed cells primed in the presence of CT-LA4Ig had a specific hyporesponsiveness when challenged with PBMC from the original donor, yet responded normally to PBMC from the third party donor. CONCLUSIONS: CTLA4Ig can inhibit T cell proliferation and induce T cell anergy in vitro by blocking B7/CD28 co-stimulatory pathway.