Effects of propofol on membrane fluidity and intracellular free Ca2+ concentration in PC12 cells / 中国药理学通报

ABSTRACT

AIM: To study the effects of propofol on membrane fluidity and intracellular free Ca2+ concentration ([Ca2+]i) in PC12 cells and discuss its relevant mechanism. METHODS: PC12 cell lines were divided into seven groups control, solvent and propofols (1, 3, 10, 30, 100 mg·L-1). Fluorescence depolarization method was used to measure dynamically microviscosity in PC12 cells and [Ca2+]i was detected using calcium fluorescentprobe Fluo-3/AM and a laser scanning confocal microscope. RESULTS: 1 Acute administration of various doses of propofol induced a significant decrease of microviscosity in PC12 cells dose-dependenty. 2 Solvent, propofol at dose of 10 mg·L-1 had no effect on [Ca2+]i in PC12 cells, however, after 30 and 100 mg·L-1 administration, [Ca2+]i increased markedly at 20-30 seconds (increase percentage were 119% and 140% respectively) and then recovered to their pre-administration levels within 50 seconds. CONCLUSION: The propofol can significantly increase membrane fluidity in PC12 cells in a dose-dependent manner and elevate [Ca2+]i in PC12 cells at doses of 30 and 100 mg·L-1. These changes are consistent with each other and related closely with anesthetic effect of propofol.