HPLC Analysis of Six Iridoid Glycosides from Cymbaria dahurica L / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a method for simultaneous determination of six iridoid glycosides from Cymbaria dahurica.METHODS: Cymbaria dahurica collected from different regions were determined by HPLC. The separation was performed on Inertsil ODS-SP column (4.6 mm×250 mm,5 μm) at 30 ℃ by gradient elution with CH3OH-H2O as the mobile phase, with the detection wavelength set at 210 nm.RESULTS: Under the chromatographic conditions adopted in this study, all the calibration curves exhibited good linearity (r>0.999 4) in a relatively wide concentration range. The recovery of the method was within the range of 95.0%-98.0%, and the RSD was less than 3%. The contents of all the compounds (S1-S6) in samples from different habitats varied significantly.CONCLUSION: The quantitative method for evaluating the quality of Cymbaria dahurica is established and can be used for the quality control of Cymbaria dahurica.